The Doerenkamp-Zbinden Chair of in-vitro
Toxicology and Biomedicine/Alternatives to Animal Experimentation

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Information Day on „Good Cell Culture Practice: human stem cells and organoids“

Information Day on „Good Cell Culture Practice: human stem cells and organoids“

 

 

Time: December 3rd 2015, from 9 am to 4 pm

 Venue: Konzil Konstanz (Council Building), Konstanz, Germany

 Registration (including Lunch):To register, please contact caat-eu@uni-konstanz.de

Until November 15th: € 350 \ € 60 (reduced price for academia) \ Free for federal agencies, students and NGO’s; After November 15th: € 500 \ € 300 (reduced price for academia) \ € 100 federal agencies, students and NGO’s;

 

Background:

The advances in cell culture techniques and the establishment of in vitro test systems in research need to be accompanied with approaches to standardize processes and documentation. The "Bologna Statement on Good Cell Culture Practice" in 1999 prompted the ECVAM Task Force on Good Cell Culture Practice (GCCP) to produce two seminal guidance documents (1-2). Additional refinements were introduced in 2011 (3).

With growing availability of cultured organoids, and the increasing generation and use of pluripotent stem cells and their differentiation progeny, there is a need for revision and update of the GCCP guidelines for these new technologies. This includes quality assurance of supplied biological materials as well as consideration of ethical and legal aspects. This symposium has the focus on GCCP regarding human cells and tissues and the quality assurance of cells obtained by reprogramming. 

1.      Hartung T, Balls M, Bardouille C, Blanck O, Coecke S, Gstraunthaler G, Lewis D; ECVAM Good Cell Culture Practice Task Force (2002).Good Cell Culture Practice. ECVAM Good Cell Culture Practice Task Force Report 1. Altern Lab Anim. 2002

2.      Coecke S, Balls M, Bowe G, Davis J, Gstraunthaler G, Hartung T, Hay R, Merten OW, Price A, Schechtman L, Stacey G, Stokes W; Second ECVAM Task Force on Good Cell Culture Practice (2005). Guidance on good cell culture practice. a report of the second ECVAM task force on good cell culture practice. Altern Lab Anim. 2005 ;33(3):261-87.

3.      Anna Bal-Price, Sandra Coeke (2011). Guidance on Good Cell Culture Practice (GCCP) in Cell Culture Techniques. Michael Aschner (ed.), Cristina Sunol (ed.) and Anna Bal-Price (ed.). Humana Press. PP 1-25

 

Agenda


09:00 - 09:15  Welcome Address

 09:15 - 09:45  Dr. Anna Bal-Price, EURL ECVAM, JRC, Italy

“Principles of Good Cell Culture Practice”

 

09:45 - 10:30  Prof. Thomas Hartung, Johns Hopkins University, CAAT. USA

University of Konstanz, CAAT-Europe, Germany

“Good Practices in modern cell culturing and organ engineering”

             10:30 - 11:00     coffee break

11:00 - 11:30  Prof. Glyn Stacey, University of Bedfordshire, UK Stem Cell Bank, UK

”Good cell Culture Practice for the routine preparation and use of stem cell-  based models incorporating examples from the SEURAT-1 Stem Cell Group”

 

11:30 - 12:00  Prof. Ulrich Martin, Leibniz Research Laboratories for Biotechnology and Artificial Organs, Hannover, Germany

“Human pluripotent stem cells for drug screening, safety pharmacology and toxicology

 12:00 - 13:00     lunch and coffee

 13:00 - 13:30  Prof. Jens Schwamborn, University of Luxembourg, Luxembourg

“Utilization of disease specific stem cells to model neurodegenerative diseases”

 13:30 - 14:00  Prof. Robert Passier, Leiden University Medical Center, The Netherlands

Human pluripotent stem cells for cardiac disease modeling and safety pharmacology

 14:00 - 14:30  Prof. Regina Grillari, Vienna Institue of Biotechnology, Austria

„Urine derived stem cells - challenges and promises“

 

14:30 - 15:00     coffee break

15:00 - 15:30  Prof. Andras Dinnyes, Biotalentum Ltd, Hungary

“Challenges in 2D and 3D neuronal differentiation of hiPSC lines”

15:30 - 16:00  Prof. Marcel Leist, University of Konstanz, CAAT-Europe, Germany

“Immortalized cell lines and their differentiation”

 

 

16:00               Closing Remarks

Prof. Thomas Hartung

 

 Presentations will be followed by a discussion to address questions from the audience